Recently, importance of carbohydrate has been increased in the cell. Carbohydrates encode information for specific molecular recognition, help determine protein folding, stability, and pharmacokinetics, play critical roles in determining biological functions, and affect diverse physiological processes. In addition, carbohydrate-protein interaction has been used to elucidate fundamental biological processes and identify new pharmaceutical substances in living cell systems. In order to fully study specific carbohydrate interactions with biomolecules such as proteins, DNA, and other carbohydrate in the cell, researchers need effective methods for carbohydrate immobilization. Carbohydrate modification and immobilization method should prove useful for diverse biomimetic studies in carbohydrates, including carbohydrate-biomolecule interaction and carbohydrate sensor or array (chip) development for diagnosis and screening.