research member publication link
본 연구실은
- 홍합접착단백질
- 항생펩타이드
- 병원성 미생물 검출 DNA칩
- 탄수화물칩
- 바이오수소
- 유기인분해생물촉매개발

Development of DNA chip for simultaneous detection of pathogenic bacteria

Food is an important energy source for our survival. However, the Centers for Disease Control and Prevention estimates that every year about 76 million people in the United States become ill from pathogens in food. Of these, up to 5,000 die. Thus, pathogenic bacteria have threatened our health and even life. Therefore, the detection and precaution of contamination by pathogenic bacteria is very important issue. Conventional method for detection of pathogenic bacteria has used culture in the selective media and characterization using identification kit. Because this method takes 3-4 days, precaution of food poisoning is impossible. To overcome this time limits, the rapid detection method using polymerize chain reaction (PCR) or enzyme linked immunosorbent assay (ELSIA) have developed. Although precaution is possible because these methods take several hours, these methods have the limitation of high-throughput analysis for precise and simultaneous detection. Here, DNA microarray is rapid, precise and high-throughput methods. This technology makes possible to get the data thousand to million experiments by an experiment of DNA microarray. Here, we checked double specific capture probes and quantitative analysis of combinatorial oligonucleotide microarray for ten pathogenic bacteria detection using 16S rDNA as detection principle. In spite of nonspecific binding, the combinatorial oligonucleotide microarray discriminated the subtype of pathogenic bacteria. Nowadays, we focused other detection principles for DNA probe, quantitative analysis and non-labeling detection using other detection system like microcantilever.

Depart of Chemical Engineering, POSTECH San 31 Hyoja-dong Nam-gu Pohang, Kyungbuk, 790-784, Korea
Tel.+82-54-279-2280(English), 5951(Korean) Fax.+82-54-279-5528 E-mail.hjcha@postech.ac.kr